論文紹介

第28回論文紹介(2016.11更新)

グループ名
分子神経生物学グループ(行動遺伝学チーム)
著者

Moonjung Hyun, Jeongho Kim, Catherine Dumur, Frank C. Schroeder, Young-Jai You


タイトル(英)
BLIMP-1/BLMP-1 and Metastasis-Associated Protein Regulate Stress Resistant Development in Caenorhabditis elegans

タイトル(日)
BLIMP-1とMTA1が線虫C. elegansのストレス環境下での発生モードを制御する
発表された専門誌
GENETICS, 203(4): 1721-1732 (2016)


Environmental stress triggers multilevel adaptations in animal development that depend in part on epigenetic mechanisms. In response to harsh environmental conditions and pheromone signals, Caenorhabditis elegans larvae become the highly stress-resistant and long-lived dauer. Despite extensive studies of dauer formation pathways that integrate specific environmental cues and appear to depend on transcriptional reprogramming, the role of epigenetic regulation in dauer development has remained unclear. Here we report that BLMP-1, the BLIMP-1 ortholog, regulates dauer formation via epigenetic pathways; in the absence of TGF-β signaling (in daf-7 mutants), lack of blmp-1 caused lethality. Using this phenotype, we screened 283 epigenetic factors, and identified lin-40, a homolog of metastasis-associate protein 1 (MTA1) as an interactor of BLMP-1. The interaction between LIN-40 and BLMP-1 is conserved because mammalian homologs for both MTA1 and BLIMP-1 could also interact. From microarray studies, we identified several downstream target genes of blmp-1npr-3, nhr-23, ptr-4, and sams-1. Of those, S-adenosyl methionine synthase (SAMS-1) is the key enzyme for production of SAM used in histone methylation. Indeed,blmp-1 is necessary for controlling histone methylation level in daf-7 mutants, suggesting BLMP-1 regulates the expression of SAMS-1, which in turn may regulate histone methylation and dauer formation. Our results reveal a new interaction between BLMP-1/BLIMP-1 and LIN-40/MTA1, as well as potential epigenetic downstream pathways, whereby these proteins cooperate to regulate stress-specific developmental adaptations.

図1:BLMP-1による発生制御モデル


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